Saturday, May 25, 2013

Parts of Light Microscope and its Function



Body Tube - It is the part of the microscope that holds the eyepiece.

Arm - The arm connects the body tube to the base.

Base - As the name suggests, the base is the lowest portion on which the whole structure of the microscope rests.

Eyepiece - It is through the eyepiece that we look at the specimen placed on the stage of the microscope. It contains two or more lenses. The most common magnification for the eyepiece is of 10x, however, they can also be of 2x and 5x.

Objective Lenses - A standard compound microscope contains two primary objective lenses, which can have magnification of 4x, 5x, 10x, 20x, 40x, 50x, and 100x. The magnification values are written on the side of each lens. The objective can be manually rotated to get the lens to give the desired magnification and focus of the specimen.

Stage - is the platform below the objective lens on which the object or specimen to be viewed is placed. There is a hole in the stage through which light beam passes and illuminates the specimen that is to be viewed.

Stage Clips - There are two stage clips, one on each side of the stage. Once the slide containing the specimen is placed on the stage, the stage clips are used to hold the slide in place.

Diaphragm - is located on the lower surface of the stage. It is used to control the amount of light that reaches the specimen through the hole in the stage.

Illuminator - Simple compound microscopes have a mirror that can be moved to adjust the amount of light that is focused on the specimen. However, some advanced types of compound microscopes have their own light source.

The Adjustments - There are two adjustment knobs, the fine adjustment knob and the coarse adjustment knob. The coarse adjustment knob helps in improving the focus at a low power, whereas the fine adjustment knob helps in adjusting the focus of the lenses with higher magnification.

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